1. Please visit the link below to download the DNA Studio for Windows:  https://dna.cellink.com/

2. Next, please download the “DNA Studio for Windows” by clicking the green button as shown below.

3. By double clicking the .exe installation file, please click "Next" as shown below:

4. Feel free to proceed with the directory installation as "C:/Program Files/CELLINK/DNA Studio" and click "Next".

5. Click "Next" to proceed.

6. Tick the license agreement radio button and click "Next".

7. Click "Next" to proceed.

8. Click "Install" to proceed. If the systems prompts something and asked "Do you want to allow this app from an unknown publisher to make changes to your device?", please feel free to tick "Yes" and the installation will commence.

9. After the successful installation, please feel free to click the "Finish" button. 

10. This will launch the application to DNA Studio. Please feel free to login with your email address and your password and click the "Sign In" button. If in case you have not yet registered, please feel free to sign up and click the "Sign Up" below.

11. After successfully signing in, the screen will show up like this as shown below. As there is no connected bioprinter as of the moment, kindly prepare the peripherals in order to connect to the software properly. Prior to plugging the peripherals, kindly click the "+" button below to add a new bioprinter.  

12. From the next screen, please feel free to name your new added printer of your choice. For this demonstration, we used "CELLINK" for instance and click "OK".

13. After following step#12, you will be able to see the newly added bioprinter in your dashboard. At this point, even if we click the "Connect" button, it won't connect because there is no peripheral that connects the laptop to your BIOX. 

14. To do so, please feel free to prepare the following:

   14a. Ethernet to Ethernet Cable 

   14b. (optional) If your laptop has no connection to Ethernet, please feel free to prepare an Ethernet-To-USB adapter as shown below:

15. In my case, I need to use an Ethernet-To-USB adapter from which I connected the USB part to my laptop while the other Ethernet cable to the BIOX.

   15a. USB cable connected to my laptop.

   15b. Ethernet cable connected to BIOX.

16. After successfully plugging the peripherals, please go back to the DNA Studio Dashboard and hit the "Connect" Button.

After clicking the "Connect" button, you will be able to see that the bioprinter is in the "Ready" state as shown below.

 

   16a. You can also verify if your bioprinter is perfectly connected to your laptop if you see this interface on your bioprinter's screen.

17. To start with, we can use a sample STL file that I got from BIOVERSE website. (For more 3D models, please feel free to visit http://bioverse.co/

 

For this demonstration, we will use Human Nose. I will also attach the STL file of this Human Nose. 

https://drive.google.com/file/d/1q_gv7T83IGZwWdqV6uDrsqiWgA4wGcBx/view?usp=sharing

 

18. To start off, please click the "Bioprint" button below:

19. From your desired directory, please double click the STL file of Human Nose. (in my case, the STL file is saved in the Desktop)


20. After selecting your STL file, you'll be able to see this interface from your DNA Studio application. By default it will show the STL file sitting on a petri dish, for instance. 

 

 

21. Next, please select the "Printheads" section, and you will shown with three printheads not yet set up. To begin with, for the purpose of demonstration, let's use the PrintHead#1 (PH1). 

22. From the dropdown menu, please tick the "Pneumatic 3ml".

 

23. At this stage, the parameters for the PrintHead#1 will be shown. 

 

24. Also, if you want to choose different infill patterns, please click the "Layers" section and choose your desired pattern.

 

25. After selecting your desired infill pattern, kindly click the "Print" button as shown below.

26. This will show the summary of the parameters we set recently as shown below. Click the "OK" button to proceed with the printing step.

27. Before we start printing, let's calibrate our bioprinter first by clicking the "Calibrate" as shown below:

28. In this screen, please feel free to calibrate by using the buttons shown in the screen (e.g. Up, Down, left, right, etc.). You may notice that while ticking the buttons, the PH1 also follows. Full sample video can be viewed from this link:

https://drive.google.com/file/d/1kSfyMSpEZqZssiFPwEbS-ufEgcppk4Ne/view?usp=sharing

29. Also, prior to starting the calibration process, please feel free to make a small dot mark (using a black whiteboard pen or any) on your print bed as a reference center for your petri dish. This way, it would be easier to calibrate using the nozzle tip by using the reference point/center. 

30. Upon pressing the Up, Down, Left and Right buttons to reach the reference center/point, the nozzle tip should appear something like this.

   30a. Also, please note that the nozzle tip should not stick too closely with the petri dish that the petri dish itself can't move freely. Please feel free to see the sample demonstration video link below that shows how the petri dish can move freely.

https://drive.google.com/file/d/1kahLebUUUIcdH2UzEg7YMGQr86txjeBP/view?usp=sharing

 

31. After successfully following steps #29 and #30, please click the "Calibrate" button as shown below, and then click "OK".

32. After successfully calibrating the system, please feel free to click the "Start" button as shown below:

33. Happy bioprinting. :)