1. Set up BIO X with desired printheads, print surface, bioinks, etc.
2. Open DNA Studio and Connect to your BIO X. (Note: Computer and BIO X should be connected using an ethernet cable).
3. Once the connection is established, select Bioprint.
4. Select desired STL file.
5. Assign the surface type in the Surface menu.
6. Assign the printer type and printer settings in the Printer menu.
7. Assign printhead number, type, and parameters in the Printheads menu.
8. You can use the Models menu to position, rotate, and scale the STL model.
9. Select the + button to add another STL file.
10. Once loaded, you can then use the Models menu to position, rotate, and scale the second model. You can select which model you want to edit by choosing your desired model from the dropdown menu. 
11. You can use the Layers tab to assign the infill pattern, density, printhead to be used, and other settings for each model file that has been loaded. Use the dropdown menu to select which model you want to edit. (Note: You can use the Layer View option to see a preview of the print toolpath).
12. When you are ready to print, select Print. 
13. Review your print parameters and select OK. 
14. Press and hold the Test Flow button to prime nozzles and test pressure settings for each printhead being used.
15. Calibrate the printer if you have not already done so. (Note: the BIO X will perform a homing sequence once Calibrate is selected). 
16. Manually calibrate printhead 1 to the desired start position (generally center of the petri dish with nozzle just touching the surface). When completed, select Calibrate. 
17. This will automatically bring you to the next printhead to calibrate if more than one printhead is set up. Repeat the same process for the next printhead making sure to move the nozzle to the exact same position (in x, y, and z) as printhead 1. Click OK when finished calibrating all printheads. 
18. Select Start to begin bioprinting!
