1: Slic3r’s settings tab: divides the software’s settings into print, filament (used only for temperature), and printer settings.
2: Settings subcategories: after clicking each general setting in area 1, different specific settings will appear for customization.
Print settings subcategory:
Allows you to adjust things such as layers and perimeters, infill, skirt and brim, support material, speed, in between others.
2.1) Layers and perimeters: allows you to adjust the height of each printed layer and the perimeter surrounding it
a) Layer height: sets the height of each layer of the object being constructed. Layer height is dependent on the nozzle diameter; as a rule of thumb 80% of the nozzle diameter should be used; however, certain material’s spread will require different thickness. Depending on the material of the selected print bed a thicker first layer should be used.
b) Vertical shells: number of times the nozzles deposits material in the perimeter, more vertical shells will create more solid walls. The spiral vase option will make the deposited material extrude non-stop, creating spiral forms. It may improve the quality of the exterior of the object.
c) Horizontal shells: will make the top and/or bottom layers more solid than the one’s inside of the object for visual purposes.
d) Quality (slower slicing): selecting the options in this subcategory will generally prevent low quality print objects and prevent errors.
e) Advanced: the seam position can influence an objects quality/water tightness. It is recommended to choose the nearest/random options in case the object needs to be water tight.
2.2) Infill: sets the pattern and density of the inner structure of the printed object. A higher percentage infill density will result in a denser object, and more used material.
a) Reducing printing time: may reduce print time by combining infill layers, adding thicker infill layers instead. It may also add infill only where it is critically needed for the structure.
b) Advanced: adds the option to print a solid infill layer every so often, modify the fill angle, which may provide better adhesion to the perimeter depending on the shape of the perimeter.
2.3) Skirt and brim: the skirt is an outline printed surrounding the area where the object is going to be printed, it allows for priming and setting a correct flow of extrude material. Adding a brim is not relevant for bio inks.
2.4) Support material: the support material is used to facilitate the printing of an object and prevent malformations in its print process. It is typically printed with a separate type of material, such as Cellink Start, that is removed after the print job is done.
a) Support material: adds the option of including support material. It also sets the overhang threshold which sets the angle slope at which support material is stopped being printed.
b) Raft: will print several layers of material between the object being printed and the print bed, possibly preventing the object from getting attached to the print bed or aid with preventing warping from the bed.
c) Options for support material and raft: sets and changes specific parameters for the support material and raft properties.
2.5) Speed: sets the speeds for specific parameters.
a) Speed for print moves: adds specific print speeds for perimeters, infills, support material and others.
b) Speed for non-print moves: changes the speed at which the nozzle travels between non-print moves.
c) Modifiers: sets the print speed of the first layer. Setting a slower speed for the first layer may improve adhesion.
d) Acceleration control (advanced): changes the acceleration between print speeds.
e) Autospeed (advanced): adjusts the maximum print speed and maximum volumetric speed.
2.6) Multiple extruders: configures the number of printheads and the material in each printhead.
a) Extruders: sets what each printhead is going to print. For example, a “1” in “Perimeter extruder” will result in printhead 1 printing the perimeter.
b) Ooze prevention: adds the option to cool unused printheads to prevent material from oozing out. Cooling temperature can also be modified.
c) Advanced: creates solid shells between changing materials. Beneficial for transparent/translucent materials, and/or support material.
2.7) Advanced: sets specific parameters such as extrusion width and infill/perimeter overlap.
a) Extrusion width: sets different width parameters for the extrusion width.
b) Overlap: ensures there is sufficient contact between the perimeter and the infill by setting the amount of overlap.
c) Flow: not relevant for bio printing.
2.8) Output options: if more than one objects are being printed at the same time, the printer has the option of completing each individual object before moving on to the next one. Instead of doing the layers of each different object at the same time.
2.9) Add notes for future remembrance.
Filament settings subcategory:
Contains the filament and cooling (not relevant for bio printing) parameters.
3.1) Filament settings are not relevant for bio printing. However, the temperature of the extruder/bed does have an impact and can be modified in this section.
Printer settings subcategory:
Sets printer configuration parameters, such as bed shape, Z offset, number of extruders, in between others.
4.1) General: contains parameters such as bed shapes and Z offset and number of extruders
a) Size and coordinates:
) Bed shape: sets the default shape and size of the print bed.
ii) Z offset: