Sample print 


  • Insert a USB flash drive with the STL or G-code file you want to print.   

  • Select Bioprintto start the printing process.  

  • Select and preview the file. 


  • Select a print surface. 

  1. Petri dish: Used for 100-mm Petri dishes. This is the recommended option for other flat substrates including paper, film and smaller dishes. This option is not limited to a 100-mm diameter print area.  

  1. Well plateUsedto print into a well plate or within a single object in an array. Tap the well plate to select specific wells to print in, or to change the order in which the wells are printed. Select Custom  to add a well plate with specific spacing or create new arrays of parts.   

  1. Glass slide:Used to constrain a print to the area of a glass slide aligned along the Y-axis in the center of the print bed. 

  • Use thPrinter menu to set the print parameters and conditions. There are several functions on this menu that can be enabled to customize the print environment:  

A. Clean Chamber fan: Turn on the Clean Chamber fan to blow sterile air. 

B. External pressure pump: Add an external air source like a compressor, gas canister or lab air 

C. Use G-code speed:Use this option to override the speed set in the Tool menuThis option is only available when a G-code file is selected.   

D. Print bed temperatureTurn on the print bed’s heating or cooling feature 

E. Import the current printer setup:Use the setup settings from the last print performed 

F.Photocrosslinking: Use the UV modules or photocuring toolhead to crosslink UV-curable materials layer-by-layer or at the end of the print. 


SelectTool 1to begin assigning print parameters.   

NOTE: Use the Printhead Selection Guide to determine which printhead is best for your specific application. The parameters used may differ depending on the selected printhead.  

  1. Tool type: Select the printhead to use from the dropdown.   

  1. Bioink profile:Select from premade or previously saved parameters 

  1. Photocrosslinking:Select a photocuring source to use with the material in Tool 1.  

  1. Nozzle diameter:Set the nozzle inner diameter in millimetersMake sure you select or enter a diameter that matches the nozzle being used.   

  1. Pressure:Set a printing pressure. If you’re using a bioink from CELLINKuse the bioink’s application note for recommendations 

  1. Pre- and postflow times: Preflow compensates for gaps in the beginning of an extruded line. Postflow compensates for materials that drip excessively after the pressure has stopped at the end of an extruded line.  

  1. Temperature: If needed, set a temperature for the printhead to maintain while printing. The printhead will not begin heating immediately. Use the Temperature function in the Tools section of the Utilities menu for preheating.   

  1. Speed: Set the speed for the printhead in millimeters per second duringextrusion. Refer the bioink’sapplication note for the recommended speeds for CELLINK’s bioinks.  

Repeat assigning these parameters for Tool 2 and Tool 3 if they are needed for the print. SelectLayers to continue.  

Use the Layers menu to input the structural characteristics of the print.  

  1. Layer profile:Select infill pattern and density from premade profile.  

  1. Perimeter:Choose which tool configured through the Printer tab to use to print the object’s exterior lines. Optionally, the exterior lines can be ignored.   

  1. Infill: Choose which tool configured through the Printer tab to use to print the object’s inner structure.   

  1. Support: Indicate that supports need to be generated for an object and specify which printhead will be used to build them. 

  1. Infill pattern: Select one of six patterns to use to generate the inner structure of an object. The output may vary from the preview based on the infill density, geometry and printing parameters.  

  1. Infill density:Select the percentage of the inner structure that should be filled with material. Subtracting the infill density from 100 will provide the porosity of an object. The spacing in the pattern is calculated based on the layer height and the nozzle diameter of the Tool selected for printing the infill.  

  1. Layer height:Specify the thickness of each layer in mm. This value cannot be greater than the diameter of the nozzle for any of the tools enabled in the Printer tab.  

  1. Preview window:Visualize the G-code generated from the parameters specified on the left. The controls for changing the view are the same as the preview window on the Model tab. 

Review the print settings and parameters, test material extrusion and save the print as a protocol that can be reused or modified later.  

  1. Dispense:Begin dispensing material at the specified pressure.  

  1. Temperature:Change the temperature of the material for the print. The current measured temperature is in parentheses.  

  1. Pressure: Change the pressure at which dispensing occurs. 

  1. Speed:Change the printhead speed.  

  1. Surface: Change the substrate used for printing.  

  1. Save:Save the print as a protocol that can be reused or modified later. 

  1. Print:Proceed to the Bioprintmenu. 

  • The options for calibrating the nozzles are automatic calibration, automatic-bed leveling and manual calibration.  

  • Automatic calibration: This method requires the least input. It is only compatible with the following printheads:  

1. 3-mL pneumatic.  

2. 10-mL pneumatic.  

3. Temperature-controlled.  

NOTE: Before running automatic calibration, autocalibration hardware must be installed and ABL probe position must be calibrated in accordance with the BIO X Autocalibration Kit Installation and Quickstart Guide. The kit is available free of charge for all BIO X customers and can be requested online 


  • The BIO X comes with disinfecting tools. The HEPA-filter fan and UV-sterilization tool can be used to clean out and disinfect the interior of the BIO X.   

  • Clean the BIO X with isopropyl alcohol, being careful to only wipe down treated metal. Anything coated with white paint is treated and can be wiped down. Do not wipe down untreated metal.  

  • Somebioinksrequire refrigeration. Always check the label of each bioinkto ensure it is stored at the temperature specified on the label. 

  • The automatic bed leveling tool is a small probe that must be stored safely when not in use.