For more information on SDS and specification sheets, see the attached documents on our website.
CELLINK LAMININK 521 has been developed as a bioink for application with cells that require the presence of the laminin 521 isoform. Laminin is an extracellular matrix protein found predominantly within the basal lamina of many tissues. This protein is characterized by its cross-like structure that serves as a structural foundation for many tissues. It consists of three domains, known as alpha, beta, and gamma. Fifteen laminin trimers exist, with each combination serving different roles and cellular compatibility. Laminin 521 consists of a chain conformation of alpha5beta2gamma1 and it plays a critical role in the activity of many cell types. This bioink retains the excellent printability and ease of crosslinking found with the universal CELLINK bioink.
CELLINK LAMININK 521 is can be utilized with several cell types and this bioink can be applicable as a great starting point for the culture of many cell types. This bioink is particularly suitable as a base material for the culture of several specific cell types, including many anchorage-dependent progenitor cell types. Furthermore, the bioink can be utilized as a base for many specialized cells including hepatocytes, cardiomyocytes, and neurons. Embryonic stem cells, mesenchymal stem cells, and induced pluripotent stem cells are compatible with this bioink. This material can be used with disease states including cancer models.
See the attached document for the bioprinting protocol.