BIO X is designed to operate between 20 and 23° Celsius, and its performance has been demonstrated at temperatures as low as -20°. 

Due to heat generated internally, the BIO X chamber temperature will rise 2-3° above room temperature while running under normal operating conditions. The running temperature is directly influenced by the temperature of the room and changing the room temperature will affect the chamber temperature. 

Humidity and moisture can negatively impact BIO X performance.